Photoelectrocyclization as an activation mechanism for organelle-specific live-cell imaging probes.

ABSTRACT: Photoactivatable fluorophores are useful tools in live-cell imaging owing to their potential for precise spatial and temporal control. In this report, a new photoactivatable organelle-specific live-cell imaging probe based on a 6π electrocyclization/oxidation mechanism is described. It is shown that this new probe is water-soluble, non-cytotoxic, cell-permeable, and useful for mitochondrial imaging. The probe displays large Stokes shifts in both pre-activated and activated forms, allowing simultaneous use with common dyes and fluorescent proteins. Sequential single-cell activation experiments in dense cellular environments demonstrate high spatial precision and utility in single- or multi-cell labeling experiments.

SUBMITTER: Tran MN 

PROVIDER: S-EPMC4485560 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature