Ontology highlight
ABSTRACT:
SUBMITTER: Bose S
PROVIDER: S-EPMC4487847 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Bose Sayantan S Wan Zhenmao Z Carr Ambrose A Rizvi Abbas H AH Vieira Gregory G Pe'er Dana D Sims Peter A PA
Genome biology 20150606
Many important biological questions demand single-cell transcriptomics on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. We report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of printing RNA on glass or capturing RNA on beads. We then develop a scalable technology for genome-wide, single-cell RNA-Seq. Our device generates pooled libraries from hundreds of individual cells wit ...[more]