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SCOPE-Seq: a scalable technology for linking live cell imaging and single-cell RNA sequencing.

ABSTRACT: Optically decodable beads link the identity of a sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and flow-based expression profiling technologies. We combine microfluidics with optically decodable beads and show that phenotypic analysis of living cells can be linked to single-cell sequencing. As a proof-of-concept, we demonstrate the accuracy and scalability of our tool called Single Cell Optical Phenotyping and Expression sequencing (SCOPE-Seq) to combine live cell imaging with single-cell RNA sequencing.

SUBMITTER: Yuan J

PROVIDER: S-EPMC6305572 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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SCOPE-Seq: a scalable technology for linking live cell imaging and single-cell RNA sequencing.

Yuan Jinzhou J Sheng Jenny J Sims Peter A PA

Genome biology 20181224 1

Optically decodable beads link the identity of a sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and flow-based expression profiling technologies. We combine microfluidics with optically decodable beads and show that phenotypic analysis of living cells can be linked to single-cell sequencing. As a proof-of-concept, we demonstrate t ...[more]

PMID: 30583733

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