Traction microscopy to identify force modulation in subresolution adhesions.
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ABSTRACT:
SUBMITTER: Han SJ
PROVIDER: S-EPMC4490115 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
Traction microscopy to identify force modulation in subresolution adhesions.
Han Sangyoon J SJ Oak Youbean Y Groisman Alex A Danuser Gaudenz G
Nature methods 20150601 7
We present a reconstruction algorithm that resolves cellular tractions in diffraction-limited nascent adhesions (NAs). The enabling method is the introduction of sparsity regularization to the solution of the inverse problem, which suppresses noise without underestimating traction magnitude. We show that NAs transmit a distinguishable amount of traction and that NA maturation depends on traction growth rate. A software package implementing this numerical approach is provided. ...[more]
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