Dataset Information

Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination.

ABSTRACT: DNA double-strand breaks (DSB) can arise during DNA replication, or after exposure to DNA-damaging agents, and their correct repair is fundamental for cell survival and genomic stability. Here, we show that the Smc5-Smc6 complex is recruited to DSBs de novo to support their repair by homologous recombination between sister chromatids. In addition, we demonstrate that Smc5-Smc6 is necessary to suppress gross chromosomal rearrangements. Our findings show that the Smc5-Smc6 complex is essential for genome stability as it promotes repair of DSBs by error-free sister-chromatid recombination (SCR), thereby suppressing inappropriate non-sister recombination events.

SUBMITTER: De Piccoli G

PROVIDER: S-EPMC4493748 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature

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Publications

Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination.

De Piccoli Giacomo G Cortes-Ledesma Felipe F Ira Gregory G Torres-Rosell Jordi J Uhle Stefan S Farmer Sarah S Hwang Ji-Young JY Machin Felix F Ceschia Audrey A McAleenan Alexandra A Cordon-Preciado Violeta V Clemente-Blanco Andrés A Vilella-Mitjana Felip F Ullal Pranav P Jarmuz Adam A Leitao Beatriz B Bressan Debra D Dotiwala Farokh F Papusha Alma A Zhao Xiaolan X Myung Kyungjae K Haber James E JE Aguilera Andrés A Aragón Luis L

Nature cell biology 20060806 9

DNA double-strand breaks (DSB) can arise during DNA replication, or after exposure to DNA-damaging agents, and their correct repair is fundamental for cell survival and genomic stability. Here, we show that the Smc5-Smc6 complex is recruited to DSBs de novo to support their repair by homologous recombination between sister chromatids. In addition, we demonstrate that Smc5-Smc6 is necessary to suppress gross chromosomal rearrangements. Our findings show that the Smc5-Smc6 complex is essential for ...[more]

PMID: 16892052

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Project description:Recombination between homologous chromosomes of different parental origin (homologs) is necessary for their accurate segregation during meiosis. It has been suggested that meiotic inter-homolog recombination is promoted by a barrier to inter-sister-chromatid recombination, imposed by meiosis-specific components of the chromosome axis. Consistent with this, measures of Holliday junction-containing recombination intermediates (joint molecules [JMs]) show a strong bias towards inter-homolog and against inter-sister JMs. However, recombination between sister chromatids also has an important role in meiosis. The genomes of diploid organisms in natural populations are highly polymorphic for insertions and deletions, and meiotic double-strand breaks (DSBs) that form within such polymorphic regions must be repaired by inter-sister recombination. Efforts to study inter-sister recombination during meiosis, in particular to determine recombination frequencies and mechanisms, have been constrained by the inability to monitor the products of inter-sister recombination. We present here molecular-level studies of inter-sister recombination during budding yeast meiosis. We examined events initiated by DSBs in regions that lack corresponding sequences on the homolog, and show that these DSBs are efficiently repaired by inter-sister recombination. This occurs with the same timing as inter-homolog recombination, but with reduced (2- to 3-fold) yields of JMs. Loss of the meiotic-chromosome-axis-associated kinase Mek1 accelerates inter-sister DSB repair and markedly increases inter-sister JM frequencies. Furthermore, inter-sister JMs formed in mek1? mutants are preferentially lost, while inter-homolog JMs are maintained. These findings indicate that inter-sister recombination occurs frequently during budding yeast meiosis, with the possibility that up to one-third of all recombination events occur between sister chromatids. We suggest that a Mek1-dependent reduction in the rate of inter-sister repair, combined with the destabilization of inter-sister JMs, promotes inter-homolog recombination while retaining the capacity for inter-sister recombination when inter-homolog recombination is not possible.

Dataset Information

Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination.

Publications

Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination.

Similar Datasets

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