Quantification of cellular viability by automated microscopy and flow cytometry.
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ABSTRACT:
SUBMITTER: Sauvat A
PROVIDER: S-EPMC4496231 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Publications
Quantification of cellular viability by automated microscopy and flow cytometry.
Sauvat Allan A Wang Yidan Y Segura Florian F Spaggiari Sabrina S Müller Kevin K Zhou Heng H Galluzzi Lorenzo L Kepp Oliver O Kroemer Guido G
Oncotarget 20150401 11
Cellular viability is usually determined by measuring the capacity of cells to exclude vital dyes such as 4',6-diamidino-2-phenylindole (DAPI), or by assessing nuclear morphology with chromatinophilic plasma membrane-permeant dyes, such as Hoechst 33342. However, a fraction of cells that exclude DAPI or exhibit normal nuclear morphology have already lost mitochondrial functions and/or manifest massive activation of apoptotic caspases, and hence are irremediably committed to death. Here, we devel ...[more]
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