Ontology highlight
ABSTRACT:
SUBMITTER: Weyand B
PROVIDER: S-EPMC4497672 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
BioResearch open access 20150501 1
We present a new method for noninvasive real-time oxygen measurement inside three-dimensional tissue-engineered cell constructs in static and dynamic culture settings in a laminar flow bioreactor. The OPAL system (optical oxygen measurement system) determines the oxygen-dependent phosphorescence lifetime of spherical microprobes and uses a two-frequency phase-modulation technique, which fades out the interference of background fluorescence from the cell carrier and culture medium. Higher cell de ...[more]