Ontology highlight
ABSTRACT:
SUBMITTER: Wulftange WJ
PROVIDER: S-EPMC6660365 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Wulftange William J WJ Rose Michelle A MA Garmendia-Cedillos Marcial M da Silva Davi D Poprawski Joanna E JE Srinivasachar Dhruv D Sullivan Taylor T Lim Langston L Bliskovsky Valery V VV Hall Matthew D MD Pohida Thomas J TJ Robey Robert W RW Morgan Nicole Y NY Gottesman Michael M MM
Journal of cellular physiology 20190422 11
Commonly used monolayer cancer cell cultures fail to provide a physiologically relevant environment in terms of oxygen delivery. Here, we describe a three-dimensional (3D) bioreactor system where cancer cells are grown in Matrigel in modified six-well plates. Oxygen is delivered to the cultures through a polydimethylsiloxane (PDMS) membrane at the bottom of the wells, with microfabricated PDMS pillars to control oxygen delivery. The plates receive 3% oxygen from below and 0% oxygen at the top su ...[more]