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ABSTRACT:
SUBMITTER: Gasparian A
PROVIDER: S-EPMC4497926 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Gasparian Alexander A Daneshian Leily L Ji Hao H Jabbari Esmaiel E Shtutman Michael M
Analytical biochemistry 20150508
Polyethylene glycol (PEG)-based hydrogels, with variable stiffness, are widely used in tissue engineering to investigate substrate stiffness effects on cell properties. Transcriptome analysis is a critical method for understanding cell physiology. However, significant RNA degradation was observed during the process of isolating and purifying RNA from cells encapsulated in the PEG hydrogel, thereby precluding purification of high-quality RNA. Here, we describe a simple protocol that prevents RNA ...[more]