Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms.
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ABSTRACT: Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-?1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an ?2-3-sialyltransferase or an ?2-6-sialyltransferase.
SUBMITTER: Li L
PROVIDER: S-EPMC4498953 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
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