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Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms.

ABSTRACT: Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-?1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an ?2-3-sialyltransferase or an ?2-6-sialyltransferase.

SUBMITTER: Li L

PROVIDER: S-EPMC4498953 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms.

Li Lei L Liu Yonghui Y Li Tiehai T Wang Wenjun W Yu Zaikuan Z Ma Cheng C Qu Jingyao J Zhao Wei W Chen Xi X Wang Peng G PG

Chemical communications (Cambridge, England) 20150601 51

Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-β1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α2-3-sialyltransferase or an α2-6-sialyltransferase. ...[more]

PMID: 26023910

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