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A fluorescent tool set for yeast Atg proteins.

ABSTRACT: Fluorescence microscopy of live cells is instrumental in deciphering the molecular details of autophagy. To facilitate the routine examination of yeast Atg proteins under diverse conditions, here we provide a comprehensive tool set, including (1) plasmids for the expression of GFP chimeras at endogenous levels for most Atg proteins, (2) RFP-Atg8 constructs with improved properties as a PAS marker, and (3) plasmids for the complementation of common yeast auxotrophic markers. We hope that the availability of this tool set will further accelerate yeast autophagy research.

SUBMITTER: Li D

PROVIDER: S-EPMC4502664 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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A fluorescent tool set for yeast Atg proteins.

Li Dan D Song Jing-Zhen JZ Shan Mei-Hua MH Li Shi-Ping SP Liu Wei W Li Hui H Zhu Jing J Wang Yue Y Lin Jianping J Xie Zhiping Z

Autophagy 20150101 6

Fluorescence microscopy of live cells is instrumental in deciphering the molecular details of autophagy. To facilitate the routine examination of yeast Atg proteins under diverse conditions, here we provide a comprehensive tool set, including (1) plasmids for the expression of GFP chimeras at endogenous levels for most Atg proteins, (2) RFP-Atg8 constructs with improved properties as a PAS marker, and (3) plasmids for the complementation of common yeast auxotrophic markers. We hope that the avai ...[more]

PMID: 25998947

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