Ontology highlight
ABSTRACT:
SUBMITTER: Park SH
PROVIDER: S-EPMC4503726 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Park Seung-Hwan SH Park Sukho S Kim Dong-Yeon DY Pyo Ayoung A Kimura Richard H RH Sathirachinda Ataya A Choy Hyon E HE Min Jung-Joon JJ Gambhir Sanjiv Sam SS Hong Yeongjin Y
PloS one 20150715 7
Monobodies are binding scaffold proteins originating from a human fibronectin domain III (Fn3) scaffold that can be easily engineered with specificity and affinity. Human EphA2 (hEphA2) is an early detection marker protein for various tumors including lung, breast, and colon cancer. In this study, we isolated two hEphA2-specific monobodies (E1 and E10) by screening a yeast surface display library. They showed the same amino acid sequence except in the DE loop and had high affinity (~2 nM Kd) aga ...[more]