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Flow Cytometry-Based Method To Detect Persisters in Candida albicans.

ABSTRACT: Candida albicans biofilms contain a subpopulation whose members are defined as persisters, displaying great tolerance of fungicides. To directly observe such persisters, an effective method using green fluorescent protein (GFP) strain labeling by mutation of the gene encoding glyceraldehyde-3-phosphate dehydrogenase (TDH3), combined with propidium iodide (PI) staining, was established. Amphotericin B-tolerant persisters harbor the characteristics of both GFP positivity [GFP (+)] and propidium iodide (PI) negativity [PI (-)], which are easily visualized using a fluorescence microscope and measured by flow cytometry.

SUBMITTER: Chang W

PROVIDER: S-EPMC4505205 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Flow Cytometry-Based Method To Detect Persisters in Candida albicans.

Chang Wenqiang W Zhang Ming M Li Ying Y Lou Hongxiang H

Antimicrobial agents and chemotherapy 20150526 8

Candida albicans biofilms contain a subpopulation whose members are defined as persisters, displaying great tolerance of fungicides. To directly observe such persisters, an effective method using green fluorescent protein (GFP) strain labeling by mutation of the gene encoding glyceraldehyde-3-phosphate dehydrogenase (TDH3), combined with propidium iodide (PI) staining, was established. Amphotericin B-tolerant persisters harbor the characteristics of both GFP positivity [GFP (+)] and propidium io ...[more]

PMID: 26014939

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