Unknown

Dataset Information

0

A Flow Cytometry-Based Serological Assay to Detect Visceral Leishmaniasis in HIV-Infected Patients.


ABSTRACT: Visceral Leishmaniasis (VL) is a severe parasitic disease that has emerged as an important opportunistic condition in HIV-infected patients and whose control is impaired by inaccurate identification. This is mainly due to the serological tests used for VL having a reduced performance in cases of VL-HIV coinfection due to a low humoral response. In this situation, however, a positive test has even greater diagnostic value when combined with the clinical status. This study aimed to evaluate the application and performance of flow cytometry to detect anti-Leishmania infantum antibodies in HIV-infected patients. Sera from VL/HIV coinfected patients, characterized using "gold standard" techniques, were compared with sera from healthy controls plus sera from HIV-infected individuals. The flow cytometry results were expressed as levels of IgG reactivity, based on the percentage of positive fluorescent parasites (PPFP). A ROC curve analysis of a serum titration indicated a PPFP of 1.26% as being the cutoff point to segregate positive and negative results. At the 1:2,048 dilution, with 89% sensitivity and 83% specificity, flow cytometry showed greater sensitivity in relation to the serological tests evaluated. Futhermore, flow cytometry was the only assay that positively identified all VL-HIV patients with quantified HIV load. Together, these findings suggest that flow cytometry may be used as an alternative serological approach for VL identification and as a tool to characterize the humoral response against Leishmania infantum in HIV-infected patients.

SUBMITTER: da Silva ED 

PROVIDER: S-EPMC8119745 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3771302 | biostudies-literature
| S-EPMC4505205 | biostudies-literature
| S-EPMC3110161 | biostudies-literature
| S-EPMC4216653 | biostudies-literature
| S-EPMC7198908 | biostudies-literature
| S-EPMC3623880 | biostudies-other
| S-EPMC7195535 | biostudies-literature
| S-EPMC8784791 | biostudies-literature
| S-EPMC6719045 | biostudies-literature
| S-EPMC6704696 | biostudies-literature