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A cross-talk between Hepatitis B virus and host mRNAs confers viral adaptation to liver.

ABSTRACT: Hepatitis B virus (HBV) chronically infects approximately 350 million people worldwide. The replication of HBV which genome is only 3.2?kb long relies heavily on host factors. Previous studies demonstrated that a highly expressed liver-specific microRNA (miRNA) miR-122 suppresses HBV expression and replication in multiple ways. In this study, we found that the miR-122 response elements in viral genome facilitate HBV expression and replication in miR-122 highly-expressed hepatocytes. Moreover, mutations in miR-122 response elements are correlated with viral loads and disease progression in HBV-infected patients. We next found that HBV mRNA with miR-122 response elements alone could lead to altered expression of multiple host genes by whole genome expression analysis. HBV mRNA-mediated miR-122 down-regulation plays a major role in HBV mRNA-induced differential gene expression. HBV mRNA could enhance viral replication via miR-122 degradation and the up-regulation of its target cyclin G1. Our study thereby reveals that under the unique condition of high abundance of miR-122 and viral mRNAs and much lower level of miR-122 target in HBV infection, HBV may have evolved to employ the miRNA-mediated virus and host mRNAs network for optimal fitness within hepatocytes.

SUBMITTER: Hu J

PROVIDER: S-EPMC4505342 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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A cross-talk between Hepatitis B virus and host mRNAs confers viral adaptation to liver.

Hu Jun J Xu Yaxing Y Li Changfei C Hao Junli J Peng Shanxin S Chu Xiaoyu X Zhang Dake D Xu Dongping D Meng Songdong S

Scientific reports 20150717

Hepatitis B virus (HBV) chronically infects approximately 350 million people worldwide. The replication of HBV which genome is only 3.2 kb long relies heavily on host factors. Previous studies demonstrated that a highly expressed liver-specific microRNA (miRNA) miR-122 suppresses HBV expression and replication in multiple ways. In this study, we found that the miR-122 response elements in viral genome facilitate HBV expression and replication in miR-122 highly-expressed hepatocytes. Moreover, mu ...[more]

PMID: 26184825

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A cross-talk between Hepatitis B virus and host mRNAs confers viral adaptation to liver.

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A cross-talk between Hepatitis B virus and host mRNAs confers viral adaptation to liver.

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