Unknown

Dataset Information

0

Facile Removal of Leader Peptides from Lanthipeptides by Incorporation of a Hydroxy Acid.


ABSTRACT: The biosynthesis of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products typically involves a precursor peptide which contains a leader peptide that is important for the modification process, and that is removed in the final step by a protease. Genome mining efforts for new RiPPs are often hampered by the lack of a general method to remove the leader peptides. We describe here the incorporation of hydroxy acids into the precursor peptides in E. coli which results in connection of the leader peptide via an ester linkage that is readily cleaved by simple hydrolysis. We demonstrate the method for two lantibiotics, lacticin 481 and nukacin ISK-1.

SUBMITTER: Bindman NA 

PROVIDER: S-EPMC4505723 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Facile Removal of Leader Peptides from Lanthipeptides by Incorporation of a Hydroxy Acid.

Bindman Noah A NA   Bobeica Silvia C SC   Liu Wenshe R WR   van der Donk Wilfred A WA  

Journal of the American Chemical Society 20150601 22


The biosynthesis of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products typically involves a precursor peptide which contains a leader peptide that is important for the modification process, and that is removed in the final step by a protease. Genome mining efforts for new RiPPs are often hampered by the lack of a general method to remove the leader peptides. We describe here the incorporation of hydroxy acids into the precursor peptides in E. coli which res  ...[more]

Similar Datasets

| S-EPMC6812601 | biostudies-literature
| S-EPMC6476326 | biostudies-literature
| S-EPMC6377482 | biostudies-literature
| S-EPMC3100556 | biostudies-literature
| S-EPMC3799897 | biostudies-literature
| S-EPMC3309271 | biostudies-literature
| S-EPMC3775851 | biostudies-literature
| S-EPMC5903816 | biostudies-literature
| S-EPMC10368454 | biostudies-literature
| S-EPMC11290303 | biostudies-literature