Improved Gene Targeting through Cell Cycle Synchronization.
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ABSTRACT:
SUBMITTER: Tsakraklides V
PROVIDER: S-EPMC4507847 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Improved Gene Targeting through Cell Cycle Synchronization.
Tsakraklides Vasiliki V Brevnova Elena E Stephanopoulos Gregory G Shaw A Joe AJ
PloS one 20150720 7
Gene targeting is a challenge in organisms where non-homologous end-joining is the predominant form of recombination. We show that cell division cycle synchronization can be applied to significantly increase the rate of homologous recombination during transformation. Using hydroxyurea-mediated cell cycle arrest, we obtained improved gene targeting rates in Yarrowia lipolytica, Arxula adeninivorans, Saccharomyces cerevisiae, Kluyveromyces lactis and Pichia pastoris demonstrating the broad applica ...[more]
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