Ontology highlight
ABSTRACT: Introduction
Several matrix metalloproteinases (MMPs) are overexpressed in lung cancer and may serve as potential targets for the development of bioactivable probes for molecular imaging.Objective
To characterize and monitor the activity of MMPs during the progression of lung adenocarcinoma.Methods
K-rasLSL-G12D mice were imaged serially during the development of adenocarcinomas using fluorescence molecular tomography (FMT) and a probe specific for MMP-2, -3, -9 and -13. Lung tumors were identified using FMT and MRI co-registration, and the probe concentration in each tumor was assessed at each time-point. The expression of Mmp2, -3, -9, -13 was quantified by qRT-PCR using RNA isolated from microdissected tumor cells. Immunohistochemical staining of overexpressed MMPs in animals was assessed on human lung tumors.Results
In mice, 7 adenomas and 5 adenocarcinomas showed an increase in fluorescent signal on successive FMT scans, starting between weeks 4 and 8. qRT-PCR assays revealed significant overexpression of only Mmp-13 in mice lung tumors. In human tumors, a high MMP-13 immunostaining index was found in tumor cells from invasive lesions (24/27), but in none of the non-invasive (0/4) (p=0.001).Conclusion
MMP-13 is detected in early pulmonary invasive adenocarcinomas and may be a potential target for molecular imaging of lung cancer.
SUBMITTER: Salaun M
PROVIDER: S-EPMC4508003 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Salaün Mathieu M Peng Jing J Hensley Harvey H HH Roder Navid N Flieder Douglas B DB Houlle-Crépin Solène S Abramovici-Roels Olivia O Sabourin Jean-Christophe JC Thiberville Luc L Clapper Margie L ML
PloS one 20150720 7
<h4>Introduction</h4>Several matrix metalloproteinases (MMPs) are overexpressed in lung cancer and may serve as potential targets for the development of bioactivable probes for molecular imaging.<h4>Objective</h4>To characterize and monitor the activity of MMPs during the progression of lung adenocarcinoma.<h4>Methods</h4>K-rasLSL-G12D mice were imaged serially during the development of adenocarcinomas using fluorescence molecular tomography (FMT) and a probe specific for MMP-2, -3, -9 and -13. ...[more]