Unknown

Dataset Information

0

MMP-2 and MMP-13 affect vasculogenic mimicry formation in large cell lung cancer.


ABSTRACT: Matrix metalloproteinases (MMPs) have critical functions in tumour vasculogenic mimicry (VM). This study explored the mechanisms underlying MMP-13 and MMP-2 regulation of tumour VM formation in large cell lung cancer (LCLC). In our study, laminin5 (Ln-5) fragments cleaved by MMP-2 promoted tubular structure formation by the LCLC cell lines H460 and H661 in three-dimensional (3D) cultures. Transient up-regulation of MMP-13 or treatment with recombinant MMP-13 protein abrogated tubular structure formation of H460 cells in 3D culture. Treated cells with Ln-5 fragments cleaved by MMP-2 stimulated EGFR and F-actin expression. Ln-5 fragments cleaved by MMP-13 decreased EGFR/F-actin expression and disrupted VM formation. MMP-13 expression was negatively correlated with VM, Ln-5 and EGFR in LCLC tissues and xenograft. In vivo experiments revealed that VM was decreased when the number of endothelium-dependent vessels (EDVs) increased during xenograft tumour growth, whereas MMP-13 expression was progressively increased. In conclusion, MMP-2 promoted and MMP-13 disrupted VM formation in LCLC by cleaving Ln-5 to influence EGFR signal activation. MMP-13 may regulate VM and EDV formation.

SUBMITTER: Li Y 

PROVIDER: S-EPMC5706571 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

MMP-2 and MMP-13 affect vasculogenic mimicry formation in large cell lung cancer.

Li Yanlei Y   Sun Baocun B   Zhao Xiulan X   Wang Xudong X   Zhang Danfang D   Gu Qiang Q   Liu Tieju T  

Journal of cellular and molecular medicine 20170802 12


Matrix metalloproteinases (MMPs) have critical functions in tumour vasculogenic mimicry (VM). This study explored the mechanisms underlying MMP-13 and MMP-2 regulation of tumour VM formation in large cell lung cancer (LCLC). In our study, laminin5 (Ln-5) fragments cleaved by MMP-2 promoted tubular structure formation by the LCLC cell lines H460 and H661 in three-dimensional (3D) cultures. Transient up-regulation of MMP-13 or treatment with recombinant MMP-13 protein abrogated tubular structure f  ...[more]

Similar Datasets

| S-EPMC5105195 | biostudies-literature
| S-EPMC6258759 | biostudies-literature
| S-EPMC8408314 | biostudies-literature
| S-EPMC7823537 | biostudies-literature
| S-EPMC8242235 | biostudies-literature
| S-EPMC4791658 | biostudies-other
| S-EPMC6050489 | biostudies-literature
| S-EPMC4907296 | biostudies-literature
2023-07-26 | GSE232214 | GEO
| S-EPMC5706568 | biostudies-literature