Ontology highlight
ABSTRACT:
SUBMITTER: Bae JH
PROVIDER: S-EPMC4508530 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Bae Jung-Hoon JH Sung Bong Hyun BH Kim Hyun-Jin HJ Park Soon-Ho SH Lim Kwang-Mook KM Kim Mi-Jin MJ Lee Cho-Ryong CR Sohn Jung-Hoon JH
Scientific reports 20150721
To produce rarely secreted recombinant proteins in the yeast Saccharomyces cerevisiae, we developed a novel genome-wide optimal translational fusion partner (TFP) screening system that involves recruitment of an optimal secretion signal and fusion partner. A TFP library was constructed from a genomic and truncated cDNA library by using the invertase-based signal sequence trap technique. The efficiency of the system was demonstrated using two rarely secreted proteins, human interleukin (hIL)-2 an ...[more]