A ratiometric threshold for determining presence of cancer during fluorescence-guided surgery.

ABSTRACT:

Background and objective

Fluorescence-guided imaging to assist in identification of malignant margins has the potential to dramatically improve oncologic surgery. However, a standardized method for quantitative assessment of disease-specific fluorescence has not been investigated. Introduced here is a ratiometric threshold derived from mean fluorescent tissue intensity that can be used to semi-quantitatively delineate tumor from normal tissue.

Methods

Open-field and a closed-field imaging devices were used to quantify fluorescence in punch biopsy tissues sampled from primary tumors collected during a phase 1 trial evaluating the safety of cetuximab-IRDye800 in patients (n = 11) undergoing surgical intervention for head and neck cancer. Fluorescence ratios were calculated usi

SUBMITTER: Warram JM 

PROVIDER: S-EPMC4510011 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature