Dataset Information

Improved Salinity Tolerance in Carrizo Citrange Rootstock through Overexpression of Glyoxalase System Genes.

ABSTRACT: Citrus plants are widely cultivated around the world and, however, are one of the most salt stress sensitive crops. To improve salinity tolerance, transgenic Carrizo citrange rootstocks that overexpress glyoxalase I and glyoxalase II genes were obtained and their salt stress tolerance was evaluated. Molecular analysis showed high expression for both glyoxalase genes (BjGlyI and PgGlyII) in 5H03 and 5H04 lines. Under control conditions, transgenic and wild type plants presented normal morphology. In salinity treatments, the transgenic plants showed less yellowing, marginal burn in lower leaves and showed less than 40% of leaf damage compared with wild type plants. The transgenic plants showed a significant increase in the dry weight of shoot but there are no differences in the root and complete plant dry weight. In addition, a higher accumulation of chlorine is observed in the roots in transgenic line 5H03 but in shoot it was lower. Also, the wild type plant accumulated around 20% more chlorine in the shoot compared to roots. These results suggest that heterologous expression of glyoxalase system genes could enhance salt stress tolerance in Carrizo citrange rootstock and could be a good biotechnological approach to improve the abiotic stress tolerance in woody plant species.

SUBMITTER: Alvarez-Gerding X

PROVIDER: S-EPMC4510252 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Publications

Improved Salinity Tolerance in Carrizo Citrange Rootstock through Overexpression of Glyoxalase System Genes.

Alvarez-Gerding Ximena X Cortés-Bullemore Rowena R Medina Consuelo C Romero-Romero Jesús L JL Inostroza-Blancheteau Claudio C Aquea Felipe F Arce-Johnson Patricio P

BioMed research international 20150708

Citrus plants are widely cultivated around the world and, however, are one of the most salt stress sensitive crops. To improve salinity tolerance, transgenic Carrizo citrange rootstocks that overexpress glyoxalase I and glyoxalase II genes were obtained and their salt stress tolerance was evaluated. Molecular analysis showed high expression for both glyoxalase genes (BjGlyI and PgGlyII) in 5H03 and 5H04 lines. Under control conditions, transgenic and wild type plants presented normal morphology. ...[more]

PMID: 26236739

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Project description:The deficit of oxygen in plants is a frequent phenomenon in nature. The main causes may be due to flooding or excessive irrigation or to an excess of aerobic microflora in the soil. The Intergovernmental Panel on Climate Change (IPCC) reported that the anthropogenically induced change of world climate increases the frequency of heavy precipitation and tropical cyclone activity. This is likely to engender more frequent flooding events in river flood plains and arable farmland, particularly affecting the world’s poorest farmers. The stress caused by lack of oxygen in plants mainly produces a reduction of the respiration, and then a low energy production and accumulation of toxic compounds of anaerobic metabolism. To alleviate this problem plants have developed morphological and metabolic adaptations, the main of which include the induction of anaerobic and fermentative pathways. In the present study we used a Combimatrix platform, made up of around 8,000 ESTs of roots isolated in citrus and its rootstocks. The chip was hybridized with RNA wild type Carrizo citrange roots and a transgenic line highly expressing a transcription factor involved in the response to the deficiency of oxygen in Oryza sativa. The aim of the work consists in the study of the up and down expressed genes in wild type and transgenic lines subjected and not to anaerobiosis, in order to understand the mechanisms that are activated under anoxia. In general, the hybridization of the chip has led to the activation of 492 genes, of which 28.95% is involved in redox processes, 28.41% in the response to stress, 18.28% in response to chemical stimuli. The up and down-regulation of differentially expressed transcripts in response to oxygen deficiency were confirmed through real-time RT-PCR The results obtained showed the activation of main genes involved in the glycolytic and fermentative pathways, such as pyruvate decarboxylase, alcohol dehydrogenase and sucrose synthase. In transgenic plants of Carrizo the response to oxygen deficit appears to be more pronounced, therefore genes of glycolytic, fermentation and pentose phosphate pathway are activated.

Dataset Information

Improved Salinity Tolerance in Carrizo Citrange Rootstock through Overexpression of Glyoxalase System Genes.

Publications

Improved Salinity Tolerance in Carrizo Citrange Rootstock through Overexpression of Glyoxalase System Genes.

Similar Datasets

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