RC3H1 post-transcriptionally regulates A20 mRNA and modulates the activity of the IKK/NF-?B pathway.

ABSTRACT: The RNA-binding protein RC3H1 (also known as ROQUIN) promotes TNF? mRNA decay via a 3'UTR constitutive decay element (CDE). Here we applied PAR-CLIP to human RC3H1 to identify ? 3,800 mRNA targets with >16,000 binding sites. A large number of sites are distinct from the consensus CDE and revealed a structure-sequence motif with U-rich sequences embedded in hairpins. RC3H1 binds preferentially short-lived and DNA damage-induced mRNAs, indicating a role of this RNA-binding protein in the post-transcriptional regulation of the DNA damage response. Intriguingly, RC3H1 affects expression of the NF-?B pathway regulators such as I?B? and A20. RC3H1 uses ROQ and Zn-finger domains to contact a binding site in the A20 3'UTR, demonstrating a not yet recognized mode of RC3H1 binding. Knockdown of RC3H1 resulted in increased A20 protein expression, thereby interfering with I?B kinase and NF-?B activities, demonstrating that RC3H1 can modulate the activity of the IKK/NF-?B pathway.

SUBMITTER: Murakawa Y 

PROVIDER: S-EPMC4510711 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature