A step toward simplified detection of serum albumin on SDS-PAGE using an environment-sensitive flavone sensor.
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ABSTRACT:
SUBMITTER: Liu B
PROVIDER: S-EPMC4512168 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
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A step toward simplified detection of serum albumin on SDS-PAGE using an environment-sensitive flavone sensor.
Liu Bin B Pang Yi Y Bouhenni Rachida R Duah Ernest E Paruchuri Sailaja S McDonald Lucas L
Chemical communications (Cambridge, England) 20150701 55
In this study, we report a series of novel flavone-based sensors that exhibit a superior fluorescence response when interacting with serum albumin in real serum samples and in acrylamide gels. The detection limit of probe 4 for serum albumin solution is 0.09 μg mL(-1), and the detectable volume for monkey serum reaches as low as 0.03 μL. ...[more]
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