A standardized model of brain death, donor treatment, and lung transplantation for studies on organ preservation and reconditioning.

ABSTRACT:

Background

We set a model of brain death, donor management, and lung transplantation for studies on lung preservation and reconditioning before transplantation.

Methods

Ten pigs (39.7?±?5.9 Kg) were investigated. Five animals underwent brain death and were treated as organ donors; the lungs were then procured and cold stored (Ischemia). Five recipients underwent left lung transplantation and post-reperfusion follow-up (Graft). Cardiorespiratory and metabolic parameters were collected. Lung gene expression of cytokines (tumor necrosis factor alpha (TNF?), interleukin-1 beta (IL-1?), interleukin-6 (IL-6), interferon gamma (IFN?), high mobility group box-1 (HMGB-1)), chemokines (chemokine CC motif ligand-2 (CCL2-MCP-1), chemokine CXC motif ligand-10 (CXCL-10), interleukin-8 (IL-8)), and endothelial activation markers (endothelin-1 (EDN-1), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), selectin-E (SELE)) was assessed by real-time polymerase chain reaction (PCR).

Results

Tachycardia and hypertension occurred during brain death induction; cardiac output rose, systemic vascular resistance dropped (P?ConclusionsWe have set a standardized, reproducible pig model resembling the entire process of organ donation that may be used as a platform to test in vivo and ex vivo strategies of donor lung optimization before transplantation.

SUBMITTER: Valenza F 

PROVIDER: S-EPMC4513016 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature