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Immunogenicity and protective efficacy of Salmonella enterica serovar Pullorum pathogenicity island 2 mutant as a live attenuated vaccine candidate.

ABSTRACT: BACKGROUND:Salmonella enterica serovar Pullorum (S. Pullorum) causes Pullorum disease (PD), a severe systemic disease of poultry and results in considerable economic losses in developing countries. In order to develop a safe and immunogenic vaccine, the immunogenicity and protective efficacy of S06004?SPI2, a Salmonella pathogenicity island 2 (SPI2) deleted mutant of S. Pullorum was evaluated in 2-day old chickens. RESULTS:Single intramuscular vaccination with S06004?SPI2 (2 × 10(7) CFU) of chickens revealed no differences in body weight or clinical symptoms compared to control group. S06004?SPI2 bacteria can colonize and persistent in liver and spleen of vaccinated chickens approximately 14 days, and specific humoral and cellular immune responses were significantly induced. Vaccination of chickens offered efficient protection against S. Pullorum strain S06004 and S. Gallinarum strain SG9 challenge, respectively, at 10 days post vaccination (dpv) based on mortality and clinical symptoms compared to control group. CONCLUSIONS:These findings suggest that S06004?SPI2 appears to be a highly immunogenic and efficient live attenuated vaccine candidate.

SUBMITTER: Yin J

PROVIDER: S-EPMC4513372 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Immunogenicity and protective efficacy of Salmonella enterica serovar Pullorum pathogenicity island 2 mutant as a live attenuated vaccine candidate.

Yin Junlei J Cheng Zhao Z Xu Lijuan L Li Qiuchun Q Geng Shizhong S Pan Zhiming Z Jiao Xinan X

BMC veterinary research 20150724

<h4>Background</h4>Salmonella enterica serovar Pullorum (S. Pullorum) causes Pullorum disease (PD), a severe systemic disease of poultry and results in considerable economic losses in developing countries. In order to develop a safe and immunogenic vaccine, the immunogenicity and protective efficacy of S06004ΔSPI2, a Salmonella pathogenicity island 2 (SPI2) deleted mutant of S. Pullorum was evaluated in 2-day old chickens.<h4>Results</h4>Single intramuscular vaccination with S06004ΔSPI2 (2 × 10( ...[more]

PMID: 26205518

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Project description:Pullorum disease is one of the most common diarrhea-related diseases caused by Salmonella enterica subspecies enterica serovar Gallinarum biovar Pullorum (S Pullorum); it negatively affects the poultry industry. However, limited studies have explored the association between the gut microbiota and S Pullorum infection in chickens. In the present study, we performed a microbiome comparison and a microbiome genome-wide association study (mGWAS) to investigate the association among the host genetics, the gut microbiota, and pullorum disease in chickens. We found that S Pullorum infection in chickens could alter the abundance of 39 bacterial genera (P < 0.05). The altered structure and composition of the gut microbiota were also detected in the offspring. mGWAS results revealed host genetic variants to be prominently associated with gut microbial diversity and individual microbes. The pathogens Pelomonas and Brevundimonas, which had a high abundance in positive parent chickens and their offspring, were significantly associated with several genetic mutations in immunity-related genes, such as TGIF1, TTLL12, and CCR7 This finding explained why Pelomonas and Brevundimonas were heritable in S Pullorum-infected chickens. The heritable gut microbes and identified genetic variants could provide references for the selection of resistant chickens and the elimination of pullorum disease.IMPORTANCE The present study investigated the association among the host genome, the gut microbiome, and S Pullorum infection in chickens. The results suggested that the gut microbial structure is altered in S Pullorum-infected chickens. The diversity and abundance of the gut microbiota remarkably differed between the offspring coming from S Pullorum-positive and S Pullorum-negative chickens. Heritable gut microbiota were detected in the offspring. Moreover, host genetic variants were associated with microbial diversity and individual gut microbes. The pathogens Pelomonas and Brevundimonas, which exhibited a high heritability in S Pullorum-positive parents and their offspring, were associated with several genetic mutations in immunity-related genes.

Dataset Information

Immunogenicity and protective efficacy of Salmonella enterica serovar Pullorum pathogenicity island 2 mutant as a live attenuated vaccine candidate.

Publications

Immunogenicity and protective efficacy of Salmonella enterica serovar Pullorum pathogenicity island 2 mutant as a live attenuated vaccine candidate.

Similar Datasets

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