Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. "2KEN8" mature pollen.
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ABSTRACT:
SUBMITTER: Zhang J
PROVIDER: S-EPMC4518142 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
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Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. "2KEN8" mature pollen.
Zhang Jin J Wu Li-Shuan LS Fan Wei W Zhang Xiao-Ling XL Jia Hui-Xia HX Li Yu Y Yin Ya-Fang YF Hu Jian-Jun JJ Lu Meng-Zhu MZ
Frontiers in plant science 20150729
Proteomic analysis was used to generate a map of Populus deltoides CL. "2KEN8" mature pollen proteins. By applying 2-D electrophoresis, we resolved 403 protein spots from mature pollen. Using the matrix-assisted laser desorption/ionization time time-of-flight/time-of-flight tandem mass spectrometry method, we identified 178 distinct proteins from 218 protein spots expressed in mature pollen. Moreover, out of these, 28 proteins were identified as putative allergens. The expression patterns of the ...[more]
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