Locking GTPases covalently in their functional states.
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ABSTRACT:
SUBMITTER: Wiegandt D
PROVIDER: S-EPMC4518245 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
Locking GTPases covalently in their functional states.
Wiegandt David D Vieweg Sophie S Hofmann Frank F Koch Daniel D Li Fu F Wu Yao-Wen YW Itzen Aymelt A Müller Matthias P MP Goody Roger S RS
Nature communications 20150716
GTPases act as key regulators of many cellular processes by switching between active (GTP-bound) and inactive (GDP-bound) states. In many cases, understanding their mode of action has been aided by artificially stabilizing one of these states either by designing mutant proteins or by complexation with non-hydrolysable GTP analogues. Because of inherent disadvantages in these approaches, we have developed acryl-bearing GTP and GDP derivatives that can be covalently linked with strategically place ...[more]
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