Dataset Information

Ghost Attractors in Spontaneous Brain Activity: Recurrent Excursions Into Functionally-Relevant BOLD Phase-Locking States.

ABSTRACT: Functionally relevant network patterns form transiently in brain activity during rest, where a given subset of brain areas exhibits temporally synchronized BOLD signals. To adequately assess the biophysical mechanisms governing intrinsic brain activity, a detailed characterization of the dynamical features of functional networks is needed from the experimental side to constrain theoretical models. In this work, we use an open-source fMRI dataset from 100 healthy participants from the Human Connectome Project and analyze whole-brain activity using Leading Eigenvector Dynamics Analysis (LEiDA), which serves to characterize brain activity at each time point by its whole-brain BOLD phase-locking pattern. Clustering these BOLD phase-locking patterns into a set of k states, we demonstrate that the cluster centroids closely overlap with reference functional subsystems. Borrowing tools from dynamical systems theory, we characterize spontaneous brain activity in the form of trajectories within the state space, calculating the Fractional Occupancy and the Dwell Times of each state, as well as the Transition Probabilities between states. Finally, we demonstrate that within-subject reliability is maximized when including the high frequency components of the BOLD signal (>0.1 Hz), indicating the existence of individual fingerprints in dynamical patterns evolving at least as fast as the temporal resolution of acquisition (here TR = 0.72 s). Our results reinforce the mechanistic scenario that resting-state networks are the expression of erratic excursions from a baseline synchronous steady state into weakly-stable partially-synchronized states - which we term ghost attractors. To better understand the rules governing the transitions between ghost attractors, we use methods from dynamical systems theory, giving insights into high-order mechanisms underlying brain function.

SUBMITTER: Vohryzek J

PROVIDER: S-EPMC7182014 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Ghost Attractors in Spontaneous Brain Activity: Recurrent Excursions Into Functionally-Relevant BOLD Phase-Locking States.

Vohryzek Jakub J Deco Gustavo G Cessac Bruno B Kringelbach Morten L ML Cabral Joana J

Frontiers in systems neuroscience 20200417

Functionally relevant network patterns form transiently in brain activity during rest, where a given subset of brain areas exhibits temporally synchronized BOLD signals. To adequately assess the biophysical mechanisms governing intrinsic brain activity, a detailed characterization of the dynamical features of functional networks is needed from the experimental side to constrain theoretical models. In this work, we use an open-source fMRI dataset from 100 healthy participants from the Human Conne ...[more]

PMID: 32362815

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Project description:BackgroundSeveral studies have reported an association between male infertility and aberrant sperm DNA methylation patterns, in particular in imprinted genes. In a recent investigation based on whole methylome and deep bisulfite sequencing, we have not found any evidence for such an association, but have demonstrated that somatic DNA contamination and genetic variation confound methylation studies in sperm of severely oligozoospermic men. To find out whether testicular germ cells (TGCs) of such patients might carry aberrant DNA methylation, we compared the TGC methylomes of four men with cryptozoospermia (CZ) and four men with obstructive azoospermia, who had normal spermatogenesis and served as controls (CTR).ResultsThere was no difference in DNA methylation at the whole genome level or at imprinted regions between CZ and CTR samples. However, using stringent filters to identify group-specific methylation differences, we detected 271 differentially methylated regions (DMRs), 238 of which were hypermethylated in CZ (binominal test, p < 2.2 × 10-16). The DMRs were enriched for distal regulatory elements (p = 1.0 × 10-6) and associated with 132 genes, 61 of which are differentially expressed at various stages of spermatogenesis. Almost all of the 67 DMRs associated with the 61 genes (94%) are hypermethylated in CZ (63/67, p = 1.107 × 10-14). As judged by single-cell RNA sequencing, 13 DMR-associated genes, which are mainly expressed during meiosis and spermiogenesis, show a significantly different pattern of expression in CZ patients. In four of these genes, the promoter is hypermethylated in CZ men, which correlates with a lower expression level in these patients. In the other nine genes, eight of which downregulated in CZ, germ cell-specific enhancers may be affected.ConclusionsWe found that impaired spermatogenesis is associated with DNA methylation changes in testicular germ cells at functionally relevant regions of the genome. We hypothesize that the described DNA methylation changes may reflect or contribute to premature abortion of spermatogenesis and therefore not appear in the mature, motile sperm.

Dataset Information

Ghost Attractors in Spontaneous Brain Activity: Recurrent Excursions Into Functionally-Relevant BOLD Phase-Locking States.

Publications

Ghost Attractors in Spontaneous Brain Activity: Recurrent Excursions Into Functionally-Relevant BOLD Phase-Locking States.

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