Ontology highlight
ABSTRACT:
SUBMITTER: Parnas O
PROVIDER: S-EPMC4522370 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Parnas Oren O Jovanovic Marko M Eisenhaure Thomas M TM Herbst Rebecca H RH Dixit Atray A Dixit Atray A Ye Chun Jimmie CJ Przybylski Dariusz D Platt Randall J RJ Tirosh Itay I Sanjana Neville E NE Shalem Ophir O Satija Rahul R Raychowdhury Raktima R Mertins Philipp P Carr Steven A SA Zhang Feng F Hacohen Nir N Regev Aviv A
Cell 20150716 3
Finding the components of cellular circuits and determining their functions systematically remains a major challenge in mammalian cells. Here, we introduced genome-wide pooled CRISPR-Cas9 libraries into dendritic cells (DCs) to identify genes that control the induction of tumor necrosis factor (Tnf) by bacterial lipopolysaccharide (LPS), a key process in the host response to pathogens, mediated by the Tlr4 pathway. We found many of the known regulators of Tlr4 signaling, as well as dozens of pre ...[more]