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Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress.

ABSTRACT: The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in coding genes, and intriguingly in microRNA genes, both involved in response to oxidative stress. These results thus suggest a profound effect of in vivo oxidative stress on the global hydroxymethylome.

SUBMITTER: Delatte B

PROVIDER: S-EPMC4523844 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress.

Delatte Benjamin B Jeschke Jana J Defrance Matthieu M Bachman Martin M Creppe Catherine C Calonne Emilie E Bizet Martin M Deplus Rachel R Marroquí Laura L Libin Myriam M Ravichandran Mirunalini M Mascart Françoise F Eizirik Decio L DL Murrell Adele A Jurkowski Tomasz P TP Fuks François F

Scientific reports 20150804

The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in co ...[more]

PMID: 26239807

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Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress

Project description:The TET-family enzymes (TETs) convert methylcytosine to hydroxymethylcytosine, a lately discovered epigenetic modification that can modulate transcription. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain. Here we show that Tet1 is sensitive to peroxide and report a global decrease in hydroxymethylcytosine in cells treated with BSO and in the intestinal epithelium of mice lacking the major antioxidant enzymes glutathione peroxidases 1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in genes involved in responses to oxidative stress. Intriguingly, a considerable proportion of these regions lie in genes encoding microRNAs predicted to target transcripts involved in oxidative stress response. This work thus demonstrates a profound effect of oxidative stress on the hydroxymethylome and opens exciting new avenues of research by highlighting a set of microRNAs that may participate in the prevention or etiology of oxidative-stress-related diseases.

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Project description:This SuperSeries is composed of the SubSeries listed below.

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