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Crystallographic analysis of FAD-dependent glucose dehydrogenase.

ABSTRACT: An FAD-dependent glucose dehydrogenase (GDH) from Aspergillus terreus was purified and crystallized at 293?K using the sitting-drop vapour-diffusion method. A data set was collected to a resolution of 1.6?Å from a single crystal at 100?K using a rotating-anode X-ray source. The crystal belonged to space group P21, with unit-cell parameters a = 56.56, b = 135.74, c = 74.13?Å, ? = 90.37°. The asymmetric unit contained two molecules of GDH. The Matthews coefficient was calculated to be 2.2?Å(3)?Da(-1) and the solvent content was estimated to be 44%.

SUBMITTER: Komori H

PROVIDER: S-EPMC4528934 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Crystallographic analysis of FAD-dependent glucose dehydrogenase.

Komori Hirofumi H Inaka Koji K Furubayashi Naoki N Honda Michinari M Higuchi Yoshiki Y

Acta crystallographica. Section F, Structural biology communications 20150728 Pt 8

An FAD-dependent glucose dehydrogenase (GDH) from Aspergillus terreus was purified and crystallized at 293 K using the sitting-drop vapour-diffusion method. A data set was collected to a resolution of 1.6 Å from a single crystal at 100 K using a rotating-anode X-ray source. The crystal belonged to space group P21, with unit-cell parameters a = 56.56, b = 135.74, c = 74.13 Å, β = 90.37°. The asymmetric unit contained two molecules of GDH. The Matthews coefficient was calculated to be 2.2 Å(3) Da( ...[more]

PMID: 26249692

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