Ontology highlight
ABSTRACT:
SUBMITTER: Yoshida H
PROVIDER: S-EPMC4642536 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Yoshida Hiromi H Sakai Genki G Mori Kazushige K Kojima Katsuhiro K Kamitori Shigehiro S Sode Koji K
Scientific reports 20150827
We report the first three-dimensional structure of fungus-derived glucose dehydrogenase using flavin adenine dinucleotide (FAD) as the cofactor. This is currently the most advanced and popular enzyme used in glucose sensor strips manufactured for glycemic control by diabetic patients. We prepared recombinant nonglycosylated FAD-dependent glucose dehydrogenase (FADGDH) derived from Aspergillus flavus (AfGDH) and obtained the X-ray structures of the binary complex of enzyme and reduced FAD at a re ...[more]