Ontology highlight
ABSTRACT:
SUBMITTER: Korogod N
PROVIDER: S-EPMC4530226 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Korogod Natalya N Petersen Carl C H CC Knott Graham W GW
eLife 20150811
Analysis of brain ultrastructure using electron microscopy typically relies on chemical fixation. However, this is known to cause significant tissue distortion including a reduction in the extracellular space. Cryo fixation is thought to give a truer representation of biological structures, and here we use rapid, high-pressure freezing on adult mouse neocortex to quantify the extent to which these two fixation methods differ in terms of their preservation of the different cellular compartments, ...[more]