Ontology highlight
ABSTRACT:
SUBMITTER: Monfort A
PROVIDER: S-EPMC4530576 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Cell reports 20150716 4
In mammals, the noncoding Xist RNA triggers transcriptional silencing of one of the two X chromosomes in female cells. Here, we report a genetic screen for silencing factors in X chromosome inactivation using haploid mouse embryonic stem cells (ESCs) that carry an engineered selectable reporter system. This system was able to identify several candidate factors that are genetically required for chromosomal repression by Xist. Among the list of candidates, we identify the RNA-binding protein Spen, ...[more]