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Large-volume en-bloc staining for electron microscopy-based connectomics.

ABSTRACT: Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM. Our protocol provides densely reconstructable tissue blocks from mouse neocortex sized at least 1 mm in diameter. By relaxing the constraints on precise topographic sample targeting, it makes the correlated functional and structural analysis of neuronal circuits realistic.

SUBMITTER: Hua Y

PROVIDER: S-EPMC4532871 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Large-volume en-bloc staining for electron microscopy-based connectomics.

Hua Yunfeng Y Laserstein Philip P Helmstaedter Moritz M

Nature communications 20150803

Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM. Our protocol provides densely reconstructable tissue blocks from mouse neocortex sized at least 1 mm in diameter. By relaxing the constraints on precise topographic sample targeting, it makes the co ...[more]

PMID: 26235643

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