Ontology highlight
ABSTRACT:
SUBMITTER: Zhou Y
PROVIDER: S-EPMC4535664 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Angewandte Chemie (Weinheim an der Bergstrasse, Germany) 20150306 17
Elaiophylin is an unusual C<sub>2</sub>-symmetric antibiotic macrodiolide produced on a bacterial modular polyketide synthase assembly line. To probe the mechanism and selectivity of diolide formation, we sought to reconstitute ring formation in vitro by using a non-natural substrate. Incubation of recombinant elaiophylin thioesterase/cyclase with a synthetic pentaketide analogue of the presumed monomeric polyketide precursor of elaiophylin, specifically its <i>N</i>-acetylcysteamine thioester, ...[more]