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Discrimination between native and Tn6010-associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by using a two-step strategy.

ABSTRACT: We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB.

SUBMITTER: Guillard T

PROVIDER: S-EPMC4538489 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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Discrimination between native and Tn6010-associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by using a two-step strategy.

Guillard Thomas T Lebreil Anne-Laure AL Hansen Lars Hestbjerg LH Kisserli Aymric A Berger Sibel S Lozniewski Alain A Alauzet Corentine C de Champs Christophe C

Antimicrobial agents and chemotherapy 20150629 9

We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB. ...[more]

PMID: 26124163

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