Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production.
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ABSTRACT:
SUBMITTER: Vuoristo KS
PROVIDER: S-EPMC4539527 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production.
Vuoristo Kiira S KS Mars Astrid E AE van Loon Stijn S Orsi Enrico E Eggink Gerrit G Sanders Johan P M JP Weusthuis Ruud A RA
Frontiers in microbiology 20150818
Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production ...[more]
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