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Labeling of fusion proteins with synthetic fluorophores in live cells.

ABSTRACT: A general approach for the sequential labeling of fusion proteins of O(6)-alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifically with different fluorophores, and the fluorescence labeling can be used for applications such as multicolor analysis of dynamic processes and fluorescence resonance energy transfer measurements. The facile access to a variety of different AGT substrates as well as the specificity of the labeling reaction should make the approach an important tool to study protein function in live cells.

SUBMITTER: Keppler A

PROVIDER: S-EPMC454197 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature

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Labeling of fusion proteins with synthetic fluorophores in live cells.

Keppler Antje A Pick Horst H Arrivoli Claudio C Vogel Horst H Johnsson Kai K

Proceedings of the National Academy of Sciences of the United States of America 20040628 27

A general approach for the sequential labeling of fusion proteins of O(6)-alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifically with different fluorophores, and the fluorescence labeling can be used for applications such as multicolor analysis of dynamic processes and fluorescence resonance energy transfer measurements. The facile access to a variety of differen ...[more]

PMID: 15226507

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