Ontology highlight
ABSTRACT:
SUBMITTER: Hinrichsen M
PROVIDER: S-EPMC6680098 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Hinrichsen M M Lenz M M Edwards J M JM Miller O K OK Mochrie S G J SGJ Swain P S PS Schwarz-Linek U U Regan L L
Protein engineering, design & selection : PEDS 20171201 12
We present a novel method to fluorescently label proteins, post-translationally, within live Saccharomycescerevisiae. The premise underlying this work is that fluorescent protein (FP) tags are less disruptive to normal processing and function when they are attached post-translationally, because target proteins are allowed to fold properly and reach their final subcellular location before being labeled. We accomplish this post-translational labeling by expressing the target protein fused to a sho ...[more]