Ontology highlight
ABSTRACT:
SUBMITTER: Ogram SA
PROVIDER: S-EPMC4542153 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Ogram Sushma A SA Boone Christopher D CD McKenna Robert R Flanegan James B JB
Virology 20140722
The mechanism of amiloride inhibition of Coxsackievirus B3 (CVB3) and poliovirus type 1 (PV1) RNA replication was investigated using membrane-associated RNA replication complexes. Amiloride was shown to inhibit viral RNA replication and VPgpUpU synthesis. However, the drug had no effect on polymerase elongation activity during either (-) strand or (+) strand synthesis. These findings indicated that amiloride inhibited the initiation of RNA synthesis by inhibiting VPg uridylylation. In addition, ...[more]