Synthetic biosensors for precise gene control and real-time monitoring of metabolites.

ABSTRACT: Characterization and standardization of inducible transcriptional regulators has transformed how scientists approach biology by allowing precise and tunable control of gene expression. Despite their utility, only a handful of well-characterized regulators exist, limiting the complexity of engineered biological systems. We apply a characterization pipeline to four genetically encoded sensors that respond to acrylate, glucarate, erythromycin and naringenin. We evaluate how the concentration of the inducing chemical relates to protein expression, how the extent of induction affects protein expression kinetics, and how the activation behavior of single cells relates to ensemble measurements. We show that activation of each sensor is orthogonal to the other sensors, and to other common inducibl

SUBMITTER: Rogers JK 

PROVIDER: S-EPMC4551912 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature