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In vivo reprogrammed pluripotent stem cells from teratomas share analogous properties with their in vitro counterparts.


ABSTRACT: Recently, induced pluripotent stem cells (iPSCs) have been generated in vivo from reprogrammable mice. These in vivo iPSCs display features of totipotency, i.e., they differentiate into the trophoblast lineage, as well as all 3 germ layers. Here, we developed a new reprogrammable mouse model carrying an Oct4-GFP reporter gene to facilitate the detection of reprogrammed pluripotent stem cells. Without doxycycline administration, some of the reprogrammable mice developed aggressively growing teratomas that contained Oct4-GFP(+) cells. These teratoma-derived in vivo PSCs were morphologically indistinguishable from ESCs, expressed pluripotency markers, and could differentiate into tissues of all 3 germ layers. However, these in vivo reprogrammed PSCs were more similar to in vitro iPSCs than ESCs and did not contribute to the trophectoderm of the blastocysts after aggregation with 8-cell embryos. Therefore, the ability to differentiate into the trophoblast lineage might not be a unique characteristic of in vivo iPSCs.

SUBMITTER: Choi HW 

PROVIDER: S-EPMC4551988 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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In vivo reprogrammed pluripotent stem cells from teratomas share analogous properties with their in vitro counterparts.

Choi Hyun Woo HW   Kim Jong Soo JS   Hong Yean Ju YJ   Song Hyuk H   Seo Han Geuk HG   Do Jeong Tae JT  

Scientific reports 20150828


Recently, induced pluripotent stem cells (iPSCs) have been generated in vivo from reprogrammable mice. These in vivo iPSCs display features of totipotency, i.e., they differentiate into the trophoblast lineage, as well as all 3 germ layers. Here, we developed a new reprogrammable mouse model carrying an Oct4-GFP reporter gene to facilitate the detection of reprogrammed pluripotent stem cells. Without doxycycline administration, some of the reprogrammable mice developed aggressively growing terat  ...[more]

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2023-08-17 | GSE174213 | GEO