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Deciphering the Non-Equivalence of Serine and Threonine O-Glycosylation Points: Implications for Molecular Recognition of the Tn Antigen by an anti-MUC1 Antibody.


ABSTRACT: The structural features of MUC1-like glycopeptides bearing the Tn antigen (?-O-GalNAc-Ser/Thr) in complex with an anti MUC-1 antibody are reported at atomic resolution. For the ?-O-GalNAc-Ser derivative, the glycosidic linkage adopts a high-energy conformation, barely populated in the free state. This unusual structure (also observed in an ?-S-GalNAc-Cys mimic) is stabilized by hydrogen bonds between the peptidic fragment and the sugar. The selection of a particular peptide structure by the antibody is thus propagated to the carbohydrate through carbohydrate/peptide contacts, which force a change in the orientation of the sugar moiety. This seems to be unfeasible in the ?-O-GalNAc-Thr glycopeptide owing to the more limited flexibility of the side chain imposed by the methyl group. Our data demonstrate the non-equivalence of Ser and Thr O-glycosylation points in molecular recognition processes. These features provide insight into the occurrence in nature of the APDTRP epitope for anti-MUC1 antibodies.

SUBMITTER: Martinez-Saez N 

PROVIDER: S-EPMC4552995 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Deciphering the Non-Equivalence of Serine and Threonine O-Glycosylation Points: Implications for Molecular Recognition of the Tn Antigen by an anti-MUC1 Antibody.

Martínez-Sáez Nuria N   Castro-López Jorge J   Valero-González Jessika J   Madariaga David D   Compañón Ismael I   Somovilla Víctor J VJ   Salvadó Míriam M   Asensio Juan L JL   Jiménez-Barbero Jesús J   Avenoza Alberto A   Busto Jesús H JH   Bernardes Gonçalo J L GJ   Peregrina Jesús M JM   Hurtado-Guerrero Ramón R   Corzana Francisco F  

Angewandte Chemie (International ed. in English) 20150626 34


The structural features of MUC1-like glycopeptides bearing the Tn antigen (α-O-GalNAc-Ser/Thr) in complex with an anti MUC-1 antibody are reported at atomic resolution. For the α-O-GalNAc-Ser derivative, the glycosidic linkage adopts a high-energy conformation, barely populated in the free state. This unusual structure (also observed in an α-S-GalNAc-Cys mimic) is stabilized by hydrogen bonds between the peptidic fragment and the sugar. The selection of a particular peptide structure by the anti  ...[more]

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