Reference gene selection for real-time quantitative PCR analysis on ovarian cryopreservation by vitrification in mice.
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ABSTRACT:
SUBMITTER: Yuanyuan S
PROVIDER: S-EPMC4554379 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
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Reference gene selection for real-time quantitative PCR analysis on ovarian cryopreservation by vitrification in mice.
Yuanyuan Shan S Qin Su S Rongrong Xu X Yujing Gao G Chengbin Pei P Jianjun Ma M Yanzhou Yang Y Xiuying Pei P
Journal of assisted reproduction and genetics 20150627 8
<h4>Purpose</h4>To ensure the correct interpretation of the results of quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) from ovarian tissue cryopreserved by vitrification, it is critical to normalize expression levels to a reference gene with stable messenger RNA (mRNA) expression in the vitrified/warmed ovarian tissue. The aim of this work was to identify suitable reference genes for qRT-PCR analysis during ovarian cryopreservation by vitrification.<h4>Methods</h ...[more]
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