Ontology highlight
ABSTRACT:
SUBMITTER: Kofoed M
PROVIDER: S-EPMC4555224 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Kofoed Megan M Milbury Karissa L KL Chiang Jennifer H JH Sinha Sunita S Ben-Aroya Shay S Giaever Guri G Nislow Corey C Hieter Philip P Stirling Peter C PC
G3 (Bethesda, Md.) 20150714 9
Systematic analyses of essential gene function using mutant collections in Saccharomyces cerevisiae have been conducted using collections of heterozygous diploids, promoter shut-off alleles, through alleles with destabilized mRNA, destabilized protein, or bearing mutations that lead to a temperature-sensitive (ts) phenotype. We previously described a method for construction of barcoded ts alleles in a systematic fashion. Here we report the completion of this collection of alleles covering 600 es ...[more]