Unknown

Dataset Information

0

Scaffold function of long non-coding RNA HOTAIR in protein ubiquitination.


ABSTRACT: Although mammalian long non-coding (lnc)RNAs are best known for modulating transcription, their post-transcriptional influence on mRNA splicing, stability and translation is emerging. Here we report a post-translational function for the lncRNA HOTAIR as an inducer of ubiquitin-mediated proteolysis. HOTAIR associates with E3 ubiquitin ligases bearing RNA-binding domains, Dzip3 and Mex3b, as well as with their respective ubiquitination substrates, Ataxin-1 and Snurportin-1. In this manner, HOTAIR facilitates the ubiquitination of Ataxin-1 by Dzip3 and Snurportin-1 by Mex3b in cells and in vitro, and accelerates their degradation. HOTAIR levels are highly upregulated in senescent cells, causing rapid decay of targets Ataxin-1 and Snurportin-1, and preventing premature senescence. These results uncover a role for a lncRNA, HOTAIR, as a platform for protein ubiquitination.

SUBMITTER: Yoon JH 

PROVIDER: S-EPMC4556280 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

altmetric image

Publications


Although mammalian long non-coding (lnc)RNAs are best known for modulating transcription, their post-transcriptional influence on mRNA splicing, stability and translation is emerging. Here we report a post-translational function for the lncRNA HOTAIR as an inducer of ubiquitin-mediated proteolysis. HOTAIR associates with E3 ubiquitin ligases bearing RNA-binding domains, Dzip3 and Mex3b, as well as with their respective ubiquitination substrates, Ataxin-1 and Snurportin-1. In this manner, HOTAIR  ...[more]

Similar Datasets

2022-02-15 | PXD029057 | Pride
| S-EPMC7281113 | biostudies-literature
| S-EPMC4383848 | biostudies-other
| S-EPMC5529952 | biostudies-other
| S-EPMC4193855 | biostudies-literature
| S-EPMC3049919 | biostudies-literature
| S-EPMC6934784 | biostudies-literature
| S-EPMC7411415 | biostudies-literature
| S-EPMC9203762 | biostudies-literature
| S-EPMC5819648 | biostudies-literature