Dataset Information

High glucose inhibits ClC-2 chloride channels and attenuates cell migration of rat keratinocytes.

ABSTRACT:

Background

Accumulating evidence has demonstrated that migration of keratinocytes is critical to wound epithelialization, and defects of this function result in chronic delayed-healing wounds in diabetes mellitus patients, and the migration has been proved to be associated with volume-activated chloride channels. The aim of the study is to investigate the effects of high glucose (HG, 25 mM) on ClC-2 chloride channels and cell migration of keratinocytes.

Methods

Newborn Sprague Dawley rats were used to isolate and culture the keratinocyte in this study. Immunofluorescence assay, real-time polymerase chain reaction, and Western blot assay were used to examine the expression of ClC-2 protein or mRNA. Scratch wound assay was used to measure the migratory ability of keratinocytes. Transwell cell migration assay was used to measure the invasion and migration of keratinocytes. Recombinant lentivirus vectors were established and transducted to keratinocytes. Whole-cell patch clamp was used to perform the electrophysiological studies.

Results

We found that the expression of ClC-2 was significantly inhibited when keratinocytes were exposed to a HG (25 mM) medium, accompanied by the decline of volume-activated Cl(-) current (I Cl,vol), migration potential, and phosphorylated PI3K as compared to control group. When knockdown of ClC-2 by RNAi or pretreatment with wortmannin, similar results were observed, including I Cl,vol and migration keratinocytes were inhibited.

Conclusion

Our study proved that HG inhibited ClC-2 chloride channels and attenuated cell migration of rat keratinocytes via inhibiting PI3K signaling.

SUBMITTER: Pan F

PROVIDER: S-EPMC4560522 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Publications

High glucose inhibits ClC-2 chloride channels and attenuates cell migration of rat keratinocytes.

Pan Fuqiang F Guo Rui R Cheng Wenguang W Chai Linlin L Wang Wenping W Cao Chuan C Li Shirong S

Drug design, development and therapy 20150828

<h4>Background</h4>Accumulating evidence has demonstrated that migration of keratinocytes is critical to wound epithelialization, and defects of this function result in chronic delayed-healing wounds in diabetes mellitus patients, and the migration has been proved to be associated with volume-activated chloride channels. The aim of the study is to investigate the effects of high glucose (HG, 25 mM) on ClC-2 chloride channels and cell migration of keratinocytes.<h4>Methods</h4>Newborn Sprague Daw ...[more]

PMID: 26355894

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Dataset Information

High glucose inhibits ClC-2 chloride channels and attenuates cell migration of rat keratinocytes.

Background

Methods

Results

Conclusion

Publications

High glucose inhibits ClC-2 chloride channels and attenuates cell migration of rat keratinocytes.

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