Ontology highlight
ABSTRACT:
SUBMITTER: Sun J
PROVIDER: S-EPMC4560639 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Sun Jim J Siroy Axel A Lokareddy Ravi K RK Speer Alexander A Doornbos Kathryn S KS Cingolani Gino G Niederweis Michael M
Nature structural & molecular biology 20150803 9
Mycobacterium tuberculosis (Mtb) induces necrosis of infected cells to evade immune responses. Recently, we found that Mtb uses the protein CpnT to kill human macrophages by secreting its C-terminal domain, named tuberculosis necrotizing toxin (TNT), which induces necrosis by an unknown mechanism. Here we show that TNT gains access to the cytosol of Mtb-infected macrophages, where it hydrolyzes the essential coenzyme NAD(+). Expression or injection of a noncatalytic TNT mutant showed no cytotoxi ...[more]